Insecticidal and antifeedant activity of Calymperes lonchophyllum Schwägr. (Bryophyta) on the fall armyworm Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae)

Resumen

Bryophytes are plants suitable for research on natural substances. Many studies have shown that they contain compounds with insecticidal and antifeedant activities. Such chemical activity is often tested through bioassays with caterpillars, especially Spodoptera Guenée larvae. The insecticidal activity by ingestion and/or antifeedant activity of the ethanolic extract of Calymperes lonchophyllum Schwägr. on 3rd-instar Spodoptera frugiperda JE Smith larvae was evaluated. Five treatments (2.0%, 1.0%, 0.5% and 0.25% μg/μl and control) with five replicates were tested, each replicate containing one larvae. Antifeedant effect and mortality were evaluated after 24, 48 and 72 hours of application of the plant extract. Antifeedant activity was observed after 48 hours of contact of the caterpillars with the treatments 1, 2 and 3. As for mortality, 72 hours of exposure to C. lonchophyllum extract at concentrations of 2.00 and 1.00 μg/μl were necessary to produce an insecticidal effect in S. frugiperda.