Computacional identification and characterization of snakins in different sugarcane (Saccharum spp.) genotypes
DOI:
https://doi.org/10.6008/CBPC2318-2881.2022.003.0003Keywords:
Sugarcane, AMP, Plant defense, In silico analysisAbstract
Sugarcane is a cultivar of great economic importance, mainly used in the production of sugar and ethanol. Through classical breeding, different cultivars are produced by crossing wild species (Saccharum officinarum and Saccharum spontaneum). In addition to the increased concentration of sucrose, the production of hybrids looks for cultivars with greater resistance to disease. Understanding the origin of resistance in cultivars is important for breeding. One of the molecules associated with plant defense is the snakins. Despite characterization in different species, snakins were not characterized in sugarcane. This work aims to identify and characterize, through bioinformatics tools, genes and transcripts in different sugarcane genotypes. We identified 13 genes in S. spontaneum; 6 genes in hybrid SP70-1143; 5 genes in hybrid R570, and 26 genes in hybrid SP80-3280, in addition to 16 transcripts. A total of 60 snakins were identified. All predicted proteins demonstrated the cysteine-rich motif, typical of snakins, in addition to new motifs. Despite the divergence of primary structures, the snakin modeling revealed high conservation of tertiary structures. Phylogenetic analysis with snakins from other plants (monocots and dicots) showed that snakins from sugarcane are dispersed. The analysis of the promoter region of the snakins genes revealed the presence of binding regions of hormone-responsive transcription factors (abscisic acid, methyl jasmonate and salicylic acid). We hope that the characterization of these important molecules will contribute to a better understanding of the different sugarcane defense responses.
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